- Machine name: unique identifier of the sequencer.
- Run number: unique number to identify the run on the sequencer.
- Lane number: positive integer (currently 1-8).
- Tile number: positive integer.
- X: x coordinate of the spot. Integer (can be negative).
- Y: y coordinate of the spot. Integer (can be negative).
- Index: positive integer. No indexing should have a value of 1.
- Read Number: 1 for single reads; 1 or 2 for paired ends.
- Sequence (BASES)
- Quality: the calibrated quality string. (QUALITIES)
- Filter: Did the read pass filtering? 0 - No, 1 - Yes.
For more information, please refer to page 6 of: ftp://ftp.era.ebi.ac.uk/meta/doc/sra_1_1/SRA_File_Formats_Guide.pdf
